The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 19, 2018

Filed:

Oct. 30, 2014
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

David R. Liu, Lexington, MA (US);

David B. Thompson, Cambridge, MA (US);

John Anthony Zuris, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/88 (2006.01); C12N 15/113 (2010.01); A61K 47/12 (2006.01); A61K 9/127 (2006.01); A61K 38/45 (2006.01); C12N 9/12 (2006.01); C12N 9/96 (2006.01); A61K 38/46 (2006.01); C07K 14/435 (2006.01); C12N 9/22 (2006.01); C07K 14/195 (2006.01); A61K 38/17 (2006.01); C07K 14/46 (2006.01); C07K 14/47 (2006.01); C12N 9/14 (2006.01); C07K 14/00 (2006.01);
U.S. Cl.
CPC ...
A61K 47/12 (2013.01); A61K 9/127 (2013.01); A61K 38/1767 (2013.01); A61K 38/45 (2013.01); A61K 38/465 (2013.01); C07K 14/00 (2013.01); C07K 14/195 (2013.01); C07K 14/43595 (2013.01); C07K 14/463 (2013.01); C07K 14/4702 (2013.01); C12N 9/1241 (2013.01); C12N 9/14 (2013.01); C12N 9/22 (2013.01); C12N 9/96 (2013.01); C07K 2319/60 (2013.01); C07K 2319/80 (2013.01); C07K 2319/85 (2013.01); C12Y 207/07 (2013.01); C12Y 301/00 (2013.01);
Abstract

Compositions, methods, strategies, kits, and systems for the delivery of negatively charged proteins, protein complexes, and fusion proteins, using cationic polymers or lipids are provided. Delivery of proteins into cells can be effected in vivo, ex vivo, or in vitro. Proteins that can be delivered using the compositions, methods, strategies, kits, and systems provided herein include, without limitation, enzymes, transcription factors, genome editing proteins, Cas9 proteins, TALEs, TALENs, nucleases, binding proteins (e.g., ligands, receptors, antibodies, antibody fragments; nucleic acid binding proteins, etc.), structural proteins, and therapeutic proteins (e.g., tumor suppressor proteins, therapeutic enzymes, growth factors, growth factor receptors, transcription factors, proteases, etc.), as well as variants and fusions of such proteins.


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