The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 12, 2018
Filed:
Oct. 06, 2015
Wafergen, Inc., Fremont, CA (US);
Syed A. Husain, Fremont, CA (US);
Bradley L. Griswold, Fremont, CA (US);
Michael Slater, Fremont, CA (US);
Patricio Espinoza, Fremont, CA (US);
Jude Dunne, Fremont, CA (US);
Glenn Hein, Fremont, CA (US);
Maithreyan Srinivasan, Fremont, CA (US);
TAKARA BIO USA, INC., Mountain View, CA (US);
Abstract
The present invention provides methods, systems, assemblies, and articles for capturing single cells with a polymer capture film. In certain embodiments, the polymer capture films comprise a plurality of individual channels with top and bottom openings, where the channels are dimensioned such that a single cell is: i) is captured inside the channel, partially or substantially occluding the channel, when negative pressure is provided to the bottom opening; or ii) is captured by the top opening, but does not enter the channel, when negative pressure is provided to the bottom opening. In some embodiments, the channels of the polymer capture film align with the wells of a multi-well chip such that the cell, or the contents of the single cell, may be transferred to a corresponding well.