The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 12, 2018

Filed:

Jan. 16, 2014
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Yu-Hwa Lo, San Diego, CA (US);

Wen Qiao, La Jolla, CA (US);

Junlan Song, La Jolla, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 27/453 (2006.01); C12N 15/10 (2006.01); B01L 3/00 (2006.01); G01N 27/447 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1003 (2013.01); B01L 3/502715 (2013.01); B01L 3/502753 (2013.01); B01L 3/502761 (2013.01); C12N 15/1013 (2013.01); C12N 15/1017 (2013.01); G01N 27/44791 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0877 (2013.01); B01L 2400/0415 (2013.01); C12Q 2565/125 (2013.01);
Abstract

Methods, systems, and devices are disclosed for capturing, concentrating, and isolating molecules in a fluid. In one aspect, a device includes a substrate formed of a material that is electrically insulating, a microfluidic channel made of an electrically insulating material formed on the substrate to carry a biofluid containing molecules including nucleic acids, an array of electrodes formed on the surface along a parallel direction of the microfluidic channel constituting a capture region, in which the array of electrodes are operable to produce an electric field across the microfluidic channel that creates an electrostatic attractive force on the nucleic acids to immobilize them in the capture region, and a chamber formed on the substrate of the electrically insulating material and connected to the microfluidic channel, the chamber configured to have a volume less than that of the microfluidic channel, in which, when the nucleic acids are released from immobilization in the capture region, the released nucleic acids are collected in the chamber.


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