The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 12, 2018

Filed:

Aug. 24, 2015
Applicant:

ES Cell International Pte Ltd, Singapore, SG;

Inventors:

Robert Passier, Leiden, NL;

Christine L. Mummery, Leiden, NL;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/071 (2010.01); C12N 5/077 (2010.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); C12Q 1/68 (2018.01); C12N 5/0735 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0657 (2013.01); G01N 33/5026 (2013.01); G01N 33/5073 (2013.01); C12N 5/0606 (2013.01); C12N 2500/25 (2013.01); C12N 2500/90 (2013.01); C12N 2500/99 (2013.01); C12N 2502/02 (2013.01); C12N 2503/02 (2013.01); C12N 2506/02 (2013.01); C12Q 1/68 (2013.01); G01N 33/5008 (2013.01); G01N 33/53 (2013.01);
Abstract

This invention provides a method for testing a factor for cardiogenicity which comprises differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence and absence of the factor wherein the human embryonic stem (hES) cells are cultured under a serum free condition comprising co-culture in the presence of END-2 cells or serum-free extracellular medium therefrom, and measuring the differentiation in the presence and absence of the factor. This invention also provides a method for identifying a cardiogenic factor, which comprises differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence or absence of the factor wherein the human embryonic stem (hES) cells are cultured under a serum free condition comprising co-culture in the presence of END-2 cells or serum-free extracellular medium therefrom, and identifying the factor that affects the differentiation of human embryonic stem (hES) cells to cardiomyocytes in the presence or absence of the factor.


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