The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 12, 2018

Filed:

Mar. 15, 2013
Applicant:

University of Washington Through Its Center for Commercialization, Seattle, WA (US);

Inventors:

Deok-Ho Kim, Seattle, WA (US);

Michael Laflamme, Seattle, WA (US);

Charles Murry, Seattle, WA (US);

Kshitiz Gupta, Seattle, WA (US);

Hyok Yoo, Shoreline, WA (US);

Alex Jiao, Seattle, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 48/00 (2006.01); C12N 5/0735 (2010.01); A61L 27/14 (2006.01); C12M 3/00 (2006.01); C12M 1/12 (2006.01); A61L 27/18 (2006.01); A61L 27/38 (2006.01); A61L 27/50 (2006.01); B01L 3/00 (2006.01); C12N 5/077 (2010.01); C12N 5/074 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0606 (2013.01); A61L 27/14 (2013.01); A61L 27/18 (2013.01); A61L 27/3826 (2013.01); A61L 27/3834 (2013.01); A61L 27/3873 (2013.01); A61L 27/50 (2013.01); B01L 3/5085 (2013.01); C12M 21/08 (2013.01); C12M 25/14 (2013.01); C12N 5/0657 (2013.01); C12N 5/0658 (2013.01); C12N 5/0696 (2013.01); A61K 48/00 (2013.01); A61L 2400/12 (2013.01); A61L 2400/18 (2013.01); B01L 2300/0851 (2013.01); B01L 2300/0896 (2013.01); B01L 2400/086 (2013.01); C12N 2503/02 (2013.01); C12N 2533/30 (2013.01); C12N 2533/40 (2013.01); C12N 2535/00 (2013.01);
Abstract

The present invention generally relates to the field of cell growth and tissue engineering, in particular, tissue engineered compositions comprising a nanotextured substrate which is structurally configured for growth of cells in an anatomically correct adult phenotype in vitro. In particular, described herein are nanotextured substrates which are structurally configured for the anisotropic organization, maturation, and growth of in vitro-differentiated muscle cells, such as cardiomyocytes, and methods for the production and use thereof in varying sizes, nanotextures and substrate rigidities. In vitro-differentiated cardiomyocytes grown on the nanotextured substrates described herein are better-differentiated and more closely mimic adult cardiac tissue than the same cells grown on a non-textured substrate of the same composition. The nanotextured substrate/cell constructs provide a platform for screening to predict the effect of test agents or drugs on, for example, human cardiac tissue, including patient-derived tissue, or for the identification of agents that effect various cardiac functional parameters.


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