The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 29, 2018

Filed:

Feb. 20, 2004
Applicants:

Teruo Okano, Ichikawa, JP;

Kohji Nishida, Ibaraki, JP;

Masayuki Yamato, Setagaya-ku, JP;

Inventors:

Teruo Okano, Ichikawa, JP;

Kohji Nishida, Ibaraki, JP;

Masayuki Yamato, Setagaya-ku, JP;

Assignees:

CELLSEED INC., Tokyo, JP;

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); A61L 27/38 (2006.01); A61L 27/50 (2006.01); C12N 5/079 (2010.01);
U.S. Cl.
CPC ...
A61L 27/3808 (2013.01); A61L 27/3839 (2013.01); A61L 27/3895 (2013.01); A61L 27/50 (2013.01); C12N 5/0621 (2013.01); A61L 2430/16 (2013.01); C12N 2539/10 (2013.01);
Abstract

An improved process for producing a regenerated corneal endothelial cell sheet, comprising the steps of allowing corneal endothelial cells collected from a tissue to be cultivated on a cell culture support having its surface covered with a polymer of which the hydrating force varies in a temperature range of 0-80° C., and after the culture, () adjusting the temperature of the culture solution to the temperature at which the polymer on the substrate surface is hydrated, () bringing the cultured corneal endothelial cell sheet into close contact with a carrier, and () detaching the sheet together with the carrier. The regenerated corneal endothelial cell sheet obtained by the process will adhere very well to living tissues.


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