Artificial dna sequence with optimized leader function in 5'(5′-utr) for the over-expression of recombinant proteins in plants and method for the production of recombinant proteins in plants

Abstract

Artificial DNA of a 5'-UTR leader region, which artificial DNA is effective in increasing the expression of recombinant proteins in plants, and comprises, along the 5′→3′ direction, an Inr initiator site and a Kozak or Kozak-like consensus sequence, and also comprises, between the Inr initiator site and the Kozak or Kozak-like consensus sequence, a plurality of poly(CAA) and a plurality of poly(CT) regions, in the same number as the poly(CAA) regions wherein at least one, optionally each one, poly(CAA) region, in the 5′→3′ direction, is upstream of a poly(CT) region and at least one poly(CAA) region, in the 5′→3′ direction, is contiguous with a poly(CT) region, wherein the artificial DNA provides the absence of A/T-rich motifs, the absence of trinucleotide elements ATT, the absence of trinucleotide elements CTG and the absence of homopolymeric tracts, that is, sequences consisting of more than 3, optionally more than 4, identical nucleotides.