The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 22, 2018

Filed:

Jul. 23, 2015
Applicant:

The Catholic University of America, Washington, DC (US);

Inventors:

Venigalla B. Rao, Silver Spring, MD (US);

Wadad Alsalmi, Washington, DC (US);

Assignee:

The Catholic University of America, Washington, DC (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/005 (2006.01); C12N 7/00 (2006.01); A61K 9/70 (2006.01); A61K 39/21 (2006.01); C07K 1/22 (2006.01); C07K 1/36 (2006.01); A61K 39/12 (2006.01); C07K 14/16 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C07K 14/005 (2013.01); A61K 9/7023 (2013.01); A61K 39/12 (2013.01); A61K 39/21 (2013.01); C07K 1/22 (2013.01); C07K 1/36 (2013.01); C12N 7/00 (2013.01); A61K 39/00 (2013.01); A61K 2039/54 (2013.01); A61K 2039/627 (2013.01); C07K 14/162 (2013.01); C07K 2319/00 (2013.01); C07K 2319/20 (2013.01); C07K 2319/21 (2013.01); C07K 2319/22 (2013.01); C07K 2319/40 (2013.01); C07K 2319/50 (2013.01); C12N 2740/16022 (2013.01); C12N 2740/16043 (2013.01); C12N 2740/16051 (2013.01); C12N 2740/16111 (2013.01); C12N 2740/16122 (2013.01); C12N 2740/16134 (2013.01); C12N 2740/16234 (2013.01);
Abstract

An approach of producing recombinant trimers that mimic native HIV-1 envelope trimers is developed. A recombinant protein forming the recombinant trimers encompasses a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140. The linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix. After expressed in a cell, the recombinant protein is secreted into the culture medium and assembles into recombinant trimers therein. The recombinant trimers may be directly purified from the culture medium. Cleaved and uncleaved trimers from different clade viruses are produced.


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