The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 22, 2018

Filed:

Oct. 30, 2014
Applicant:

Osaka University, Osaka, JP;

Inventor:

Hiroshi Egusa, Osaka, JP;

Assignee:

OSAKA UNIVERSITY, Osaka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); A61L 27/36 (2006.01); A61K 35/32 (2015.01); C12N 5/074 (2010.01); A61L 27/54 (2006.01); A61K 38/39 (2006.01); A61K 33/06 (2006.01); A61K 35/28 (2015.01); A61K 35/545 (2015.01); A61L 27/12 (2006.01); A61L 27/24 (2006.01); C12N 5/077 (2010.01); A61L 27/38 (2006.01);
U.S. Cl.
CPC ...
A61L 27/3683 (2013.01); A61K 33/06 (2013.01); A61K 35/28 (2013.01); A61K 35/32 (2013.01); A61K 35/545 (2013.01); A61K 38/39 (2013.01); A61L 27/12 (2013.01); A61L 27/24 (2013.01); A61L 27/365 (2013.01); A61L 27/3633 (2013.01); A61L 27/3691 (2013.01); A61L 27/3834 (2013.01); A61L 27/54 (2013.01); C12N 5/0654 (2013.01); C12N 5/0696 (2013.01); A61L 2300/412 (2013.01); A61L 2300/64 (2013.01); A61L 2400/06 (2013.01); A61L 2430/02 (2013.01); C12N 2500/14 (2013.01); C12N 2506/45 (2013.01); C12N 2533/54 (2013.01);
Abstract

Provided is a bone regeneration agent comprising an inactivated cell construct derived from stem cells as a source material, the inactivated cell construct at least containing a mineral and an extracellular matrix. The bone regeneration agent has osteoconductive and osteoinductive abilities, and is inexpensively producible and size-controllable. Also provided is a method comprising the steps of: (1) inducing differentiation of stem cells into mineral-producing cells in agitated culture under the condition of 0.01 to 1.00 Hz to give a cell aggregate at least containing a mineral and an extracellular matrix, or (1') inducing differentiation of stem cells genetically engineered to overexpress a protein associated with bone formation into mineral-producing cells in agitated or static culture, to give a cell aggregate at least containing a mineral and an extracellular matrix, and(2) inactivating the cell aggregate obtained in the preceding step. The method enables easy production of the above-mentioned bone regeneration agent.


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