The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 15, 2018
Filed:
Feb. 19, 2009
Applicants:
Hans Huber, Vienna, AT;
Gerhard Weigl, Amstetten, AT;
Wolfgang Buchinger, Vienna, AT;
Inventors:
Assignee:
BOEHRINGER INGELHEIM RCV GMBH & CO KG, Vienna, AT;
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 1/20 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 1/20 (2013.01); C12N 15/1003 (2013.01); C12P 19/34 (2013.01);
Abstract
A process for producing plasmid DNAcells comprises a pre-culture and fed-batch process. The culture media of the batch phase and the culture medium added during the feeding phase are chemically defined. The culture medium of the feeding phase contains a growth-limiting substrate and is added, for at least a fraction of the feeding phase, at a feeding rate that follows a pre-defined exponential function, thereby controlling the specific growth rate at a pre-defined value. The process results in high yield and homogeneity of plasmid DNA.