The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 15, 2018
Filed:
Mar. 04, 2009
Applicants:
Michael Tovey, Paris, FR;
Christophe Lallemand, Paris, FR;
Inventors:
Michael Tovey, Paris, FR;
Christophe Lallemand, Paris, FR;
Assignees:
LE CENTRE NATIONALE DE LA RECHERCHE SCIENTIFIQUE, Paris, FR;
BIOMONITOR LIMITED, Galway, IE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/00 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01); C12Q 1/68 (2018.01); C07K 16/24 (2006.01);
U.S. Cl.
CPC ...
C07K 16/249 (2013.01); G01N 33/5041 (2013.01); G01N 33/6872 (2013.01); C07K 2317/76 (2013.01);
Abstract
The present invention provides a cell for use in a one-step cell-based assay for an extracellular ligand (e.g., IFNα) that initiates a ligand-specific signal at the nucleus of the cell and for neutralizing antibodies against the extracellular ligand. The cell-based one-step assay allows both the extracellular ligand concentration and the neutralizing antibody titer to be quantified in a single sample (e.g., serum) without the need for sample dilution and addition of exogenous extracellular ligand.