The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 08, 2018
Filed:
May. 08, 2017
Colorado State University Research Foundation, Fort Collins, CO (US);
Kallie C. Kessler, Denver, CO (US);
Eric L. Patterson, Fort Collins, CO (US);
Margaret B. Fleming, Fort Collins, CO (US);
Todd A. Gaines, Fort Collins, CO (US);
Colorado State University Research Foundation, Fort Collins, CO (US);
Abstract
Methods are provided for high throughput genotyping of plants, utilizing at least three primers, one primer recognizing a polymorphic sequence of a first species of a plant genus, a second primer recognizing a second polymorphic sequence of a second species, and a third primer that recognizes sequences of both the first and second species and producing a measurable signal when amplifying a plant DNA-containing sample. Additional primers recognizing additional species may also be employed. The method may be repeated for multiple sequences each diagnosing a species or hybrid, and results analyzed using data from multiple assays to improve the statistical robustness of genotyping results. Controls are provided in which the primer target sequences are introduced into and extracted from bacteria and the measurable signal used as a control. The methods are particularly useful for genotyping a population of plants, especially where weed species and/or hybrids are present.