The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 08, 2018

Filed:

Oct. 16, 2013
Applicant:

Roche Molecular System, Inc., Pleasanton, CA (US);

Inventors:

Frank Bergmann, Iffeldorf, DE;

Dorothea Sizmann, Iffeldorf, DE;

Heike Zitzer, Weinheim, DE;

Assignee:

Roche Molecular Systems, Inc., Pleasanton, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 19/34 (2006.01); C12Q 1/70 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6813 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/707 (2013.01);
Abstract

The present invention relates to a method for amplifying and detecting a target nucleic acid in a sample, said target nucleic comprising subgroups with sequence variations and/or individual mutations, wherein an amplification of the nucleic acids in said sample is carried out. This amplification involves a polymerase, primers for generating an amplicon and at least two detectable probes specific for different sequence portions of said amplicon. Detection of the obtained amplicon is brought about by detecting hybridization of the probes mentioned above to said different sequence portions of the amplicon. The invention further provides reaction mixtures and kits for amplifying and detecting a target nucleic acid comprising subgroups with sequence variations and/or individual mutations involving the use of at least two detectable probes specific for different sequence portions of an amplicon.


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