The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 08, 2018
Filed:
Aug. 04, 2015
Yang Shao, Nanjing, CN;
Yang Shao, Nanjing, CN;
NANJING SHIHE GENE BIOTECHNOLOGY INC., LTD., Nanjing, CN;
Abstract
This application provides a detailed description of a washing solution consisting of saline sodium citrate (SSC) buffer and sodium dodecyl sulfate (SDS) for hybridization-enrichment-capture DNA sequencing libraries as well as a corresponding washing method. The invented solution has a simple recipe, which is low-cost and easily produced and stored. The washing strategy associated with the invented solution is simple and easy to carry out, requiring no special instruments, and the results produced thereby are outstanding with low background noise, which consequently leads to an improvement on the efficiency of targeted enrichment. Elution of the library after washing is easy to carry out and requires no additional reagents or purification, effectively reducing loss of material due to further purification and time needed for completion of procedure.