The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 08, 2018

Filed:

Dec. 29, 2014
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Jennifer A. Doudna, Oakland, CA (US);

Samuel H. Sternberg, Oakland, CA (US);

Martin Jinek, Oakland, CA (US);

Fuguo Jiang, Oakland, CA (US);

Emine Kaya, Oakland, CA (US);

David W. Taylor, Jr., Oakland, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); C12N 15/10 (2006.01); G06F 19/16 (2011.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); C12N 15/1031 (2013.01); C12Y 301/00 (2013.01); G06F 19/16 (2013.01); C07K 2299/00 (2013.01);
Abstract

The present disclosure provides atomic structures of Cas9 with and without polynucleotides bound thereto. Also provided is a computer-readable medium comprising atomic coordinates for Cas9 polypeptides in both an unbound configuration and a configuration wherein the Cas9 polypeptide is bound to one or more polynucleotides. The present disclosure provides crystals comprising Cas9 polypeptides; and compositions comprising the crystals. The present disclosure provides methods for the engineering of Cas9 polypeptides wherein Cas9 activity has been altered, ablated, or preserved and amended with additional activities.


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