The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 08, 2018

Filed:

Dec. 21, 2016
Applicant:

Qiagen Sciences, Llc, Waltham, MA (US);

Inventors:

Michel Georges Perbost, Belmont, MA (US);

Thomas Daniel Perroud, Lexington, MA (US);

Assignee:

QIAGEN SCIENCES, LLC, Waltham, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); G01N 35/08 (2006.01); G01N 35/00 (2006.01); B01D 69/02 (2006.01); G01N 35/10 (2006.01); C12N 15/10 (2006.01); B01J 19/00 (2006.01); G01N 15/14 (2006.01); C12M 3/06 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502761 (2013.01); B01D 69/02 (2013.01); B01L 3/5027 (2013.01); B01L 3/50273 (2013.01); G01N 35/08 (2013.01); B01D 2325/06 (2013.01); B01D 2325/08 (2013.01); B01J 19/0046 (2013.01); B01L 3/50857 (2013.01); B01L 7/52 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/1822 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/086 (2013.01); C12M 23/16 (2013.01); C12N 15/1017 (2013.01); G01N 15/1484 (2013.01); G01N 35/1011 (2013.01); G01N 2015/149 (2013.01);
Abstract

A flowcell for a sequencing instrument. The flowcell includes a fluid inlet, a fluid outlet, a flow channel formed between an at least partially transparent cover and a base and fluidly connecting the fluid inlet to the fluid outlet, and a capture substrate provided in the flow channel. The capture substrate includes microretainers configured to each receive a single microspot having a microspot diameter, and microretainer is separated from adjacent microretainers by an interstitial gap distance that is equal to or greater than the microspot diameter. A particle separator may be fluidly connected to the flowcell. The particle separator may include a microfluidic channel having an array of micropillars to transfer a plurality of the microspots to a loading buffer that may be delivered to the flowcell.


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