The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 01, 2018

Filed:

Mar. 15, 2013
Applicant:

Nugen Technologies, Inc., San Carlos, CA (US);

Inventors:

Christopher Armour, Kirkland, WA (US);

Doug Amorese, Los Altos, CA (US);

Bin Li, Palo Alto, CA (US);

Nurith Kurn, Palo Alto, CA (US);

Assignee:

NUGEN TECHNOLOGIES, INC., San Carlos, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12N 15/1093 (2013.01); C12N 15/1096 (2013.01); C12Q 1/6844 (2013.01);
Abstract

The present invention provides methods, compositions and kits for the generation of next generation sequencing (NGS) libraries in which non-desired nucleic acid sequences have been depleted or substantially reduced. The methods, compositions and kits provided herein are useful, for example, for the production of libraries from total RNA with reduced ribosomal RNA and for the reduction of common mRNA species in expression profiling from mixed samples where the mRNAs of interest are present at low levels. The methods of the invention can be employed for the elimination of non-desired nucleic acid sequences in a sequence-specific manner, and consequently, for the enrichment of nucleic acid sequences of interest in a nucleic acid library.


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