The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 24, 2018

Filed:

May. 06, 2011
Applicants:

James Hickman, Orlando, FL (US);

Xiufang Guo, Oviedo, FL (US);

Mercedes Gonzalez, Casselberry, FL (US);

Maria Stancescu, Oviedo, FL (US);

Inventors:

James Hickman, Orlando, FL (US);

Xiufang Guo, Oviedo, FL (US);

Mercedes Gonzalez, Casselberry, FL (US);

Maria Stancescu, Oviedo, FL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/07 (2010.01); C12N 5/071 (2010.01); C12N 5/0793 (2010.01); C12N 5/0797 (2010.01); C12N 5/0735 (2010.01); C12N 5/0789 (2010.01); G01N 33/50 (2006.01); G01N 33/487 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5058 (2013.01); C12N 5/0697 (2013.01); G01N 33/48728 (2013.01); G01N 33/6872 (2013.01); G01N 33/6887 (2013.01); C12N 2500/90 (2013.01); C12N 2501/01 (2013.01); C12N 2501/105 (2013.01); C12N 2501/13 (2013.01); C12N 2501/385 (2013.01); C12N 2501/41 (2013.01); C12N 2501/58 (2013.01); C12N 2502/081 (2013.01); C12N 2502/1335 (2013.01); C12N 2533/20 (2013.01); C12N 2535/10 (2013.01); G01N 2500/10 (2013.01);
Abstract

A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more rat muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a rat muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.


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