The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 24, 2018

Filed:

Jan. 21, 2014
Applicant:

Promega Corporation, Madison, WI (US);

Inventors:

Brock Binkowski, Sauk City, WI (US);

Lance P. Encell, Fitchburg, WI (US);

Mary Hall, Waunakee, WI (US);

Matthew B. Robers, Madison, WI (US);

Michael R. Slater, Madison, WI (US);

Keith V. Wood, Mount Horeb, WI (US);

Monika G. Wood, Mount Horeb, WI (US);

Assignee:

Promega Corporation, Madison, WI (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/02 (2006.01); C12N 1/21 (2006.01); C12N 15/00 (2006.01); C12Q 1/66 (2006.01); C07D 487/04 (2006.01); C12N 15/52 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/66 (2013.01); C07D 487/04 (2013.01); C12N 9/0004 (2013.01); C12N 9/0069 (2013.01); C12N 15/52 (2013.01); C12Y 113/12005 (2013.01); C12Y 113/12007 (2013.01); A01K 2217/072 (2013.01);
Abstract

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-typeluciferase.


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