The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 24, 2018

Filed:

Mar. 06, 2014
Applicants:

The General Hospital Corporation, Boston, MA (US);

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Cammie Lesser, Cambridge, MA (US);

Amy Jo Wagers, Cambridge, MA (US);

Analise Z. Reeves, Brookline, MA (US);

Assignees:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/70 (2006.01); C12N 15/74 (2006.01); A61K 35/74 (2015.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/70 (2013.01); A61K 35/74 (2013.01); C12N 15/74 (2013.01); A61K 48/00 (2013.01);
Abstract

The methods and compositions described herein relate to the delivery of polypeptides to a target cell, e.g. by utilizing engineered non-pathogenic bacteria comprising a type three secretion system (T3SS) and T3SS-compatible substrates. In one aspect, described herein are non-pathogenic microbial cells that have been engineered to express both a functional type three secretion system (T3SS) and at least one polypeptide that is compatible with the T3SS. Due to the wide variety of polypeptides that can be delivered to a target eukaryotic cell using the compositions and systems described herein, a commensurately wide variety of applications is contemplated, e.g. therapeutics and reprogramming.


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