The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 24, 2018

Filed:

Apr. 01, 2015
Applicant:

Healthgen Biotechnology Co., Ltd., Wuhan, Hubei, CN;

Inventors:

Daichang Yang, Hubei, CN;

Yang He, Hubei, CN;

Guangfei Li, Hubei, CN;

Jingru Liu, Hubei, CN;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01J 20/00 (2006.01); C07K 14/765 (2006.01); C07K 1/18 (2006.01); B01J 20/32 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
C07K 1/18 (2013.01); B01J 20/3285 (2013.01); C07K 14/765 (2013.01);
Abstract

A method for separating and purifying recombinant human serum albumin (rHSA) from transgenic rice grain, sequentially comprising the steps of: 1) subjecting crude extract of rHSA to cation exchange chromatography to obtain primary product I; 2) subjecting the primary product I to anion exchange chromatography to obtain secondary product II; 3) subjecting the secondary product II to hydrophobic chromatography to obtain purified rHSA. The method may further comprise a step of ceramic hydroxyapatite chromatography prior to the hydrophobic chromatography. The method has the advantages of low cost and easy operation. The resultant rHSA has a purity of about 99% by HPLC.


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