The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 17, 2018

Filed:

Jun. 10, 2011
Applicant:

Ichiro Sase, Tokyo, JP;

Inventor:

Ichiro Sase, Tokyo, JP;

Assignee:

NIKON CORPORATION, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G02B 21/16 (2006.01); G01N 21/64 (2006.01); G02B 21/36 (2006.01); G02B 21/06 (2006.01); G02B 27/56 (2006.01);
U.S. Cl.
CPC ...
G02B 21/16 (2013.01); G01N 21/6458 (2013.01); G02B 21/367 (2013.01); G02B 21/06 (2013.01); G02B 27/56 (2013.01);
Abstract

A microscope apparatus comprises: a distribution measurement apparatus which—with respect to an observation region wherein a sample is arranged which comprises a fluorescent material that is activated when irradiated with an activating light of a prescribed wavelength, and that emits fluorescence when irradiated in an activated condition with an exciting light of a wavelength that differs from that of the activating light—obtains a fluorescent picture image by conducting irradiation with the exciting light, and measures a fluorescent intensity distribution of the observation region; an irradiation intensity setting apparatus which sets irradiation intensities of the activating light for respective portions of the observation region based on the fluorescent intensity distribution; and a picture image formation apparatus which obtains a plurality of the fluorescent picture images by multiply repeating operations comprising an operation wherein the observation region is irradiated with the activating light at the irradiation intensities that have been set in the respective portions, and an operation wherein a fluorescent picture image is obtained by irradiating the observation region with the exciting light after the activating light irradiation, and which generates a sample picture image from the plurality of the fluorescent picture images.


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