The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 17, 2018

Filed:

May. 30, 2011
Applicants:

Mattias Algotsson, Uppsala, SE;

Lars C. Andersson, Uppsala, SE;

Eggert Brekkan, Uppsala, SE;

Lee Hedemyr, Uppsala, SE;

Jean-luc Maloisel, Uppsala, SE;

Ronnie Palmgren, Uppsala, SE;

Inventors:

Mattias Algotsson, Uppsala, SE;

Lars C. Andersson, Uppsala, SE;

Eggert Brekkan, Uppsala, SE;

Lee Hedemyr, Uppsala, SE;

Jean-Luc Maloisel, Uppsala, SE;

Ronnie Palmgren, Uppsala, SE;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07C 237/10 (2006.01); C08B 37/00 (2006.01); B01J 20/289 (2006.01); B01J 20/32 (2006.01); C07C 323/60 (2006.01); B01J 20/281 (2006.01);
U.S. Cl.
CPC ...
C08B 37/0039 (2013.01); B01J 20/281 (2013.01); B01J 20/289 (2013.01); B01J 20/3219 (2013.01); B01J 20/3251 (2013.01); B01J 20/3265 (2013.01); C07C 237/10 (2013.01); C07C 323/60 (2013.01);
Abstract

The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity. The dimeric pentadentate chelator according to the invention has the following general formula:


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