The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 10, 2018

Filed:

Oct. 19, 2015
Applicant:

Molecular Devices, Llc, Sunnyvale, CA (US);

Inventors:

Matthew Chan, Palo Alto, CA (US);

Nia W. Fong, Menlo Park, CA (US);

Robert Malcolm Watson, Jr., Castro Valley, CA (US);

Assignee:

Molecular Devices, LLC, San Jose, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G02B 15/22 (2006.01); G02B 21/24 (2006.01); G02B 21/16 (2006.01); G02B 21/08 (2006.01); G01N 21/64 (2006.01); G02B 7/38 (2006.01); G02B 21/36 (2006.01); G02B 21/26 (2006.01);
U.S. Cl.
CPC ...
G02B 21/244 (2013.01); G01N 21/6452 (2013.01); G01N 21/6458 (2013.01); G02B 7/38 (2013.01); G02B 21/086 (2013.01); G02B 21/16 (2013.01); G02B 21/26 (2013.01); G02B 21/367 (2013.01);
Abstract

Microscope system for, and methods of, imaging a sample including biological cells. In an exemplary method, light transmitted through the sample may be detected for a first set of focal positions to collect a first stack of images. Values of a focus metric may be calculated for the first stack of images. A candidate focal position may be determined based on the values. Photoluminescence may be detected from the sample for a second set of focal positions to collect a second stack of images. The second set of focal positions may define a smaller range than the first set of focal positions. At least one focal position of the second set of focal positions may be based on the candidate focal position. In other words, the candidate focal position may serve as a guide for finding a suitable photoluminescence focal position.


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