The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 10, 2018
Filed:
Jul. 16, 2012
Fazoil Inoyatovich Ataullakhanov, Moscow, RU;
Nataljya Mikhajlovna Dashkevich, Pyschino, RU;
Mikhail Vladimirovich Ovanesov, Zelenograd, RU;
Vasilii Ivanovich Sarbash, Moscow, RU;
Mikhail Aleksandrovich Panteleev, Mytishchi, RU;
Sergey Sergeevich Karamzin, Moscow, RU;
Andrey Yurjevich Kondratovich, Moscow, RU;
Fazoil Inoyatovich Ataullakhanov, Moscow, RU;
Nataljya Mikhajlovna Dashkevich, Pyschino, RU;
Mikhail Vladimirovich Ovanesov, Zelenograd, RU;
Vasilii Ivanovich Sarbash, Moscow, RU;
Mikhail Aleksandrovich Panteleev, Mytishchi, RU;
Sergey Sergeevich Karamzin, Moscow, RU;
Andrey Yurjevich Kondratovich, Moscow, RU;
Abstract
The invention relates to the field of biotechnology. The method for determining the spatial and temporal distribution of the activity of a proteolytic enzyme in an in vitro heterogeneous system, such as blood or blood plasma, involves the introduction of a luminescent, fluorogenic or chromogenic substrate into a sample with the subsequent release of a detectable tag as the proteolytic enzyme cleaves the substrate, and the recording of the optical characteristics of the sample, which makes it possible to assess the spatial and temporal distribution of the activity of the enzyme. The device for the implementation of the above method comprises an in vitro system, a means for illuminating the sample, a recording means and a control means. A method for diagnosing homeostatic imbalances according to a change in the spatial and temporal distribution of the activity of a proteolytic enzyme in a blood sample is also proposed.