The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 10, 2018
Filed:
Sep. 09, 2015
Applicant:
Jnc Corporation, Tokyo, JP;
Inventor:
Satoshi Inouye, Kanagawa, JP;
Assignee:
JNC CORPORATION, Tokyo, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12N 9/02 (2006.01);
U.S. Cl.
CPC ...
C12N 15/85 (2013.01); C12N 9/0069 (2013.01); C12Y 113/12 (2013.01); C12Y 113/12005 (2013.01); C12Y 113/12007 (2013.01); C12Y 113/12013 (2013.01); C12N 2800/22 (2013.01);
Abstract
The present invention provides a method of designing an optimized gene which comprises altering a nucleotide sequence of a target protein gene, so that only preferential codons with high frequency of use in human cells are selected and a GC content of not less than 60% is achieved. A gene design method which involves the feature 'only preferential codons with high frequency of use are selected and a GC content of not less than 60% is achieved' can be established as a general rule for preparing proteins with high expression level, in order to obtain chemically synthesized genes for proteins capable of high-level expression in eukaryotes.