The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 10, 2018

Filed:

Nov. 22, 2013
Applicants:

Consejo Superior DE Investigaciones Científicas (Csic), Madrid, ES;

Instituto Nacional DE Técnica Aeroespacial “esteban Terradas”, Torrejón de Ardoz, Madrid, ES;

Inventors:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/115 (2010.01); C12N 15/10 (2006.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
C12N 15/115 (2013.01); C12N 15/1048 (2013.01); G01N 33/56988 (2013.01); C12N 2310/16 (2013.01); C12N 2310/3519 (2013.01); C12N 2320/30 (2013.01); G01N 2469/10 (2013.01);
Abstract

The invention relates to an aptamer, the structure thereof comprising at least one nucleotide sequence 5'-GGCA(A/G)GGA-3′, that can specifically bind to the poly(A) hairpin of the 5′UTR region of the genome of the human immunodeficiency virus type 1 (HIV-1), providing the method for producing aptamers with said sequence by means of a combination of experimental techniques of in vitro selection of nucleic acids with computational techniques of sequence optimization. The invention also relates to a DNA gene structure for synthesizing said aptamers, preferably RNA. The invention further relates to the different uses of the above-mentioned aptamer, including the use thereof as a biosensor molecule for detecting and/or quantifying HIV-1, as an inhibitor of the production of viral particles of HIV-1, and to the application thereof in medicine, the invention also relating to a method for treating a disease caused by HIV-1, and to a pharmaceutical composition comprising said aptamer.


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