The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 03, 2018

Filed:

Apr. 04, 2013
Applicant:

Becton, Dickinson and Company, Franklin Lakes, NJ (US);

Inventors:

Xiao Li, Germantown, MD (US);

Yongqiang Zhang, Lutherville, MD (US);

Kenneth Anthony Kopher, Baltimore, MD (US);

John D. Mantlo, Westminster, MD (US);

William Alfred Pope, Owings Mills, MD (US);

Song Shi, Reisterstown, MD (US);

Axel A. Yup, Owings Mills, MD (US);

Assignee:

Becton, Dickinson and Company, Franklin Lakes, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); G01N 1/30 (2006.01); C12Q 1/06 (2006.01); C12Q 1/04 (2006.01);
U.S. Cl.
CPC ...
G01N 1/30 (2013.01); C12Q 1/04 (2013.01); C12Q 1/06 (2013.01); G01N 2001/302 (2013.01);
Abstract

Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.


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