The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 03, 2018

Filed:

May. 24, 2012
Applicants:

David T. Wong, Beverly Hills, CA (US);

Fang Wei, North Hills, CA (US);

Wei Liao, North Hills, CA (US);

Inventors:

David T. Wong, Beverly Hills, CA (US);

Fang Wei, North Hills, CA (US);

Wei Liao, North Hills, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12N 15/10 (2006.01); G01N 33/50 (2006.01); G01N 33/543 (2006.01); G01N 33/569 (2006.01); G01N 33/49 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6881 (2013.01); C12N 15/1013 (2013.01); C12Q 1/6806 (2013.01); G01N 33/5005 (2013.01); G01N 33/5076 (2013.01); G01N 33/54326 (2013.01); G01N 33/56966 (2013.01); G01N 33/49 (2013.01);
Abstract

The molecules harbored in exosomes play important roles in biological science. A highly desirable goal for exosome research is the rapid, simple, simultaneous tracking and quantification of exosome harbored molecules. Disclosed herein are methods and devices for inducing the release and measurement of biomolecules harbored in exosomes. The disclosed method, Electric Field Induced Release and Measurement (EFIRM) technique, uses an electrical field to simultaneously disrupt exosomes to release the contents and measure the harbored exosomal RNA/proteins. The exosome vesicle contents can be released within minutes. This provides a potential on-site method for the detection of exosome-harbored biomolecules.


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