The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 03, 2018

Filed:

Dec. 18, 2014
Applicants:

Kelvin Lee, Newark, DE (US);

Abraham Lenhoff, Newark, DE (US);

Kristin Valente, Plymouth Meeting, PA (US);

Nick Levy, Philadelphia, PA (US);

Yatin Gokarn, San Diego, CA (US);

Inventors:

Kelvin Lee, Newark, DE (US);

Abraham Lenhoff, Newark, DE (US);

Kristin Valente, Plymouth Meeting, PA (US);

Nick Levy, Philadelphia, PA (US);

Yatin Gokarn, San Diego, CA (US);

Assignee:

University of Delaware, Newark, DE (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); C12N 15/113 (2010.01); C07K 16/00 (2006.01); C12P 21/02 (2006.01); C12N 9/20 (2006.01); A61K 38/17 (2006.01); A61K 38/39 (2006.01); A61K 38/42 (2006.01); A61K 38/44 (2006.01); A61K 38/45 (2006.01); A61K 38/48 (2006.01); A61K 38/52 (2006.01); A61K 47/26 (2006.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1137 (2013.01); A61K 38/1709 (2013.01); A61K 38/1741 (2013.01); A61K 38/1761 (2013.01); A61K 38/1774 (2013.01); A61K 38/39 (2013.01); A61K 38/42 (2013.01); A61K 38/44 (2013.01); A61K 38/443 (2013.01); A61K 38/45 (2013.01); A61K 38/482 (2013.01); A61K 38/488 (2013.01); A61K 38/4813 (2013.01); A61K 38/52 (2013.01); A61K 47/26 (2013.01); C07K 16/00 (2013.01); C12N 9/20 (2013.01); C12P 21/005 (2013.01); C12P 21/02 (2013.01); C12N 2310/10 (2013.01); C12N 2310/14 (2013.01); C12N 2310/20 (2017.05); C12Y 104/03013 (2013.01); C12Y 111/01015 (2013.01); C12Y 205/01018 (2013.01); C12Y 207/04006 (2013.01); C12Y 207/11001 (2013.01); C12Y 301/01034 (2013.01); C12Y 304/16005 (2013.01); C12Y 304/21 (2013.01); C12Y 304/23005 (2013.01); C12Y 502/01008 (2013.01); C12Y 503/04001 (2013.01);
Abstract

The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof.


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