The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 03, 2018

Filed:

May. 11, 2012
Applicants:

Minoru S. H. Ko, Cockeysville, MD (US);

Tetsuya Hirata, Minato-ku, JP;

Inventors:

Minoru S. H. Ko, Cockeysville, MD (US);

Tetsuya Hirata, Minato-ku, JP;

Assignee:

ELIXIRGEN, LLC, Baltimore, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 15/00 (2006.01); C12N 15/63 (2006.01); C07H 21/04 (2006.01); C07K 1/00 (2006.01); C12N 5/074 (2010.01); C12N 15/85 (2006.01); C12N 5/0735 (2010.01); C12N 15/79 (2006.01); C07K 14/47 (2006.01); C12N 15/86 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0696 (2013.01); C12N 15/85 (2013.01); C07H 21/04 (2013.01); C07K 14/47 (2013.01); C12N 5/0606 (2013.01); C12N 15/63 (2013.01); C12N 15/79 (2013.01); C12N 15/86 (2013.01); C12N 2501/60 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2501/605 (2013.01); C12N 2501/606 (2013.01); C12N 2501/608 (2013.01); C12N 2506/00 (2013.01); C12N 2506/1307 (2013.01);
Abstract

Disclosed herein is the finding that Zscan4 is an early embryonic factor that facilitates cellular reprogramming. In particular, Zscan4 can replace the oncogenic reprogramming factor c-Myc to produce induced pluripotent stem cells when co-expressed with Klf4, Oct4 and Sox2. In addition, several Zscan4-dependent genes were identified that promote iPSC formation when co-expressed with known reprogramming factors. Thus, the present disclosure provides an ex vivo method of producing an iPS cell by reprogramming of a somatic cell. The method includes contacting the somatic cell with a Zscan4, or a Zscan4-dependent gene, and at least one reprogramming factor. Also provided are iPS cells produced by the disclosed method and non-human animals generated from such iPS cells.


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