The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 27, 2018
Filed:
Mar. 16, 2015
Applicant:
Hoffmann-la Roche Inc., Little Falls, NJ (US);
Inventor:
Christian Schantz, Penzberg, DE;
Assignee:
HOFFMANN-LA ROCHE INC., Little Falls, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/21 (2006.01); C12P 21/00 (2006.01); C07K 1/14 (2006.01); C12P 21/02 (2006.01); C07K 14/47 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C07K 1/145 (2013.01); C07K 14/21 (2013.01); C07K 14/47 (2013.01); C12P 21/02 (2013.01);
Abstract
Herein is reported a method for producing a polypeptide comprising the step of incubating (resuspended) prokaryotic cells in a solution comprising about 10 mM to about 95 mM Tris-HCl and about 2 mM to about 6 mM EDTA at a pH value of about 7 to about 10 for about 15 min to about 6 h at about 25° C.