The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 20, 2018

Filed:

Feb. 21, 2013
Applicant:

The Trustees of the University of Pennsylvania, Philadelphia, PA (US);

Inventors:

Dennis E. Discher, Philadelphia, PA (US);

Jae-Won Shin, Philadelphia, PA (US);

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); A61K 35/19 (2015.01); C12N 5/0789 (2010.01); C12N 5/078 (2010.01); C12Q 1/68 (2006.01); A61K 35/28 (2015.01); G01N 1/10 (2006.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
C12M 47/04 (2013.01); A61K 35/19 (2013.01); A61K 35/28 (2013.01); C12N 5/0644 (2013.01); C12N 5/0647 (2013.01); C12Q 1/6881 (2013.01); G01N 1/10 (2013.01); G01N 33/56972 (2013.01); C12N 2501/998 (2013.01);
Abstract

The present invention relates to a bioreactor apparatus, and methods of use, for the isolation of rare blood cells, including hematopoietic stem cells and megakaryocytes. The apparatus includes a soft substrate and an anti-contractility agent, thereby providing a soft microenvironment to cultured cells. The apparatus of the invention is permissive for the survival of non-dividing cells while dividing cells are eliminated. This unique property allows for the simple isolation of rare blood cells without the use of costly equipment and antibodies.


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