The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 06, 2018
Filed:
Nov. 27, 2013
Applicant:
Larix Biosciences Llc, Sunnyvale, CA (US);
Inventors:
Bo Yu, Sunnyvale, CA (US);
James Larrick, Sunnyvale, CA (US);
Assignee:
Larix Bioscience, LLC, Sunnyvale, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6897 (2018.01); C12N 15/63 (2006.01); G01N 33/569 (2006.01); C07K 16/00 (2006.01); C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C07K 16/28 (2006.01);
U.S. Cl.
CPC ...
G01N 33/56966 (2013.01); C07K 16/00 (2013.01); C07K 16/2887 (2013.01); C12N 15/1086 (2013.01); C12N 15/63 (2013.01); C12Q 1/6897 (2013.01); C07K 2317/14 (2013.01);
Abstract
The invention provides a novel cell line development method useful to screen for recombinant protein production. The method utilizes a membrane-anchored reporter or an intracellular reporter residing in the expression vector for a gene of interest to facilitate initial cell selection by FACS or MACS. A switching mechanism can be used to delete the reporter from the chromosome by providing an appropriate DNA recombinase, which turns the selected cells into production cells that secrete the protein of interest without co-expression of the reporter.