The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 06, 2018
Filed:
Jun. 01, 2007
Albert Bosch Navarro, Barcelona, ES;
Rosa Maria Pinto Sole, Barcelona, ES;
Albert Bosch Navarro, Barcelona, ES;
Rosa Maria Pinto Sole, Barcelona, ES;
UNIVERSIDAD DE BARCELONA, Barcelona, ES;
Abstract
A method for the accurate quantification of a virus in a sample, by reverse transcription polymerase chain reaction (RT-PCR), includes: adding a known concentration of a Mengo virus to the sample as control for the nucleic acids extraction step, the Mengo virus being a mutant strain with the same growth properties than those of the wild-type Mengo virus, and with non-pathogenic capacity; performing a nucleic acids extraction to obtain a nucleic acids suspension; analyzing the nucleic acids suspension by RT-PCR with primers and probes; quantifying the amplimers resulting from the RT-PCR; determining the concentration of the virus in the sample by comparison of the value obtained with an appropriate standard curve; and determining the concentration of the Mengo virus by comparison of the value obtained with an appropriate standard curve.