The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 06, 2018
Filed:
Mar. 21, 2016
Integrated Micro-chromatography Systems, Columbia, SC (US);
Jia Yang, Columbia, SC (US);
Gary Horvath, Columbia, SC (US);
Pongkwan Sitasuwan, Columbia, SC (US);
Margarita Marinova, Columbia, SC (US);
Qian Wang, Columbia, SC (US);
Lim Andrew Lee, Columbia, SC (US);
INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS, Irmo, SC (US);
Abstract
Mutatedstrain 269Y β-glucuronidase enzymes with enhanced enzymatic activity at low pH (e.g., below pH 6.8), as well as enhanced thermostability as compared to wild type enzyme are provided. The enzymes of the invention advantageously allow for accurate analysis of bodily samples for the presence of drugs at low pH and in 30 minutes or less, as compared to the several hours needed using prior enzyme preparations. Methods of using the mutated enzymes for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided.