The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 27, 2018

Filed:

Jun. 26, 2015
Applicant:

Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Inventors:

David Frendewey, New York, NY (US);

Gustavo Droguett, New City, NY (US);

Anthony Gagliardi, Hopewell Junction, NY (US);

Junko Kuno, Holmes, NY (US);

Wojtek Auerbach, Ridgewood, NJ (US);

David M. Valenzuela, Yorktown Heights, NY (US);

Assignee:

Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/90 (2006.01); C12N 5/0735 (2010.01); C12N 15/85 (2006.01); A01K 67/027 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8509 (2013.01); A01K 67/0275 (2013.01); A01K 67/0276 (2013.01); A01K 67/0278 (2013.01); C12N 5/0606 (2013.01); C12N 15/907 (2013.01); A01K 2217/075 (2013.01); A01K 2227/105 (2013.01); A01K 2267/02 (2013.01); A01K 2267/0393 (2013.01); C12N 2500/60 (2013.01); C12N 2517/00 (2013.01);
Abstract

Methods and compositions are provided for generating targeted genetic modifications on the Y chromosome or a challenging target locus. Compositions include an in vitro culture comprising an XY pluripotent and/or totipotent animal cell (i.e., XY ES cells or XY iPS cells) having a modification that decreases the level and/or activity of an Sry protein; and, culturing these cells in a medium that promotes development of XY F0 fertile females. Such compositions find use in various methods for making a fertile female XY non-human mammal in an F0 generation.


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