The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 27, 2018

Filed:

May. 02, 2013
Applicants:

Illumina, Inc., San Diego, CA (US);

Illumina Cambridge Limited, Nr Saffron Walden, GB;

Inventors:

Eric H. Kawashima, Pacific Grove, CA (US);

Laurent Farinelli, Plan-les-Ouates, CH;

Pascal Mayer, Saint-Beauzire, FR;

Assignees:

Illumina, Inc., San Diego, CA (US);

Illumina Cambridge Limited, Little Chesterford, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C07H 21/00 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1065 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01);
Abstract

A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provide further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.


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