The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 27, 2018
Filed:
Jun. 05, 2013
Cj Healthcare Corporation, Seoul, KR;
Alteogen, Inc, Daejeon, KR;
Gil Bu Kang, Busan, KR;
Chung Min Lee, Gyeonggi-do, KR;
Jee Won Ahn, Seoul, KR;
Dong Eok Lee, Seoul, KR;
Chang Bong Jun, Seoul, KR;
Won Jeong Lee, Seoul, KR;
Sung Yoo Cho, Gyeonggi-do, KR;
Chi Hye Park, Gyeonggi-do, KR;
Ki Wan Kim, Seoul, KR;
Yoon Jung Lee, Gyeonggi-do, KR;
Ji Hyun Moon, Seoul, KR;
Hoo Keun Oh, Seoul, KR;
Young Joon Park, Seoul, KR;
Sang Hyun Lee, Seoul, KR;
Hyoung Taek Lim, Incheon, KR;
Soon Jae Park, Daejeon, KR;
Hye Shin Chung, Daejeon, KR;
Sang Mee Lee, Daejeon, KR;
CJ HEALTHCARE CORPORATION, Seoul, KR;
ALTEOGEN, INC, Daejeon, KR;
Abstract
The present invention relates to a long-acting human growth hormone NexP-hGH protein and its production method. More specifically, it relates to a specific isoform of long-acting human growth hormone NexP-hGH protein in which human growth hormone is fused with a highly glycosylated alpha-1 antitrypsin mutant whereby long-acting properties in vivo are increased. The present invention also relates to a high-purity purification method for NexP-hGH, which includes the steps of: (a) carrying out anion-exchange resin chromatography on a biological emulsion comprising NexP-hGH in which human growth hormone is fused with an alpha-1 antitrypsin mutant; (b) carrying out hydrophobic resin chromatography on the biological emulsion comprising NexP-hGH, or on the eluate produced in step (a); and (c) carrying out affinity chromatography, entailing packing with a resin to which anti-alpha-1 antitrypsin antibody fragments are attached, on the biological emulsion comprising NexP-hGH and on the eluate produced in step (a) or step (b).