The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 27, 2018

Filed:

Sep. 30, 2016
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Jonathan Schultz, Guilford, CT (US);

John Nobile, Guilford, CT (US);

Brian Reed, Woodbridge, CT (US);

Prasanna Thwar, Los Altos, CA (US);

Todd Roswech, Westbrook, CT (US);

John Andrew Sheridan, Marblehead, MA (US);

Assignee:

Life Technologies Corporation, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); C12M 3/00 (2006.01); C12M 1/34 (2006.01); B01F 3/08 (2006.01); C12P 19/34 (2006.01); C12M 1/26 (2006.01); B01F 15/00 (2006.01); B04B 11/02 (2006.01); B01F 5/04 (2006.01); B01F 13/00 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); B04B 5/04 (2006.01); B04B 7/02 (2006.01); C12Q 1/68 (2018.01);
U.S. Cl.
CPC ...
B01F 3/0807 (2013.01); B01F 5/0485 (2013.01); B01F 13/0059 (2013.01); B01F 15/00 (2013.01); B01L 3/5021 (2013.01); B01L 7/52 (2013.01); B04B 5/0414 (2013.01); B04B 7/02 (2013.01); B04B 11/02 (2013.01); C12M 33/10 (2013.01); C12P 19/34 (2013.01); C12Q 1/686 (2013.01); B01F 2215/0037 (2013.01); B01L 3/502784 (2013.01); B01L 2200/026 (2013.01); B01L 2300/046 (2013.01); B01L 2300/06 (2013.01); B01L 2300/18 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1827 (2013.01); B01L 2300/1838 (2013.01); B01L 2400/0409 (2013.01); B04B 2007/025 (2013.01);
Abstract

An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.


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