The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 20, 2018
Filed:
Jul. 22, 2015
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
Romualdas Vaisvila, Ipswich, MA (US);
Heidi E. Johnson, Ipswich, MA (US);
Saulius Vainauskas, Newburyport, MA (US);
Theodore B. Davis, Boxford, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/78 (2006.01); C12Q 1/68 (2006.01); C12N 9/02 (2006.01); C12N 9/12 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12N 9/0071 (2013.01); C12N 9/1252 (2013.01); C12N 9/78 (2013.01); C12Q 1/6827 (2013.01); C12Y 114/11 (2013.01); C12Y 207/07007 (2013.01); C12Y 305/04005 (2013.01);
Abstract
Compositions and methods are provided for discrimination between cytosine and modifications thereof using cytidine deaminases and/or oxygenases. Variants of wild type cytidine deaminases are described which show reduced bias with respect to adjacent nucleotides upstream of the cytosine. The methods provide a rapid and convenient use of enzymes to obtain methylomes.