The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 13, 2018

Filed:

Oct. 15, 2010
Applicants:

Mark W. Eshoo, Solana Beach, CA (US);

John Picuri, San Diego, CA (US);

Curtis Phillipson, San Diego, CA (US);

Inventors:

Mark W. Eshoo, Solana Beach, CA (US);

John Picuri, San Diego, CA (US);

Curtis Phillipson, San Diego, CA (US);

Assignee:

IBIS BIOSCIENCES, INC., Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/64 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2018.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12Q 1/6844 (2013.01);
Abstract

The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample.


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