The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 13, 2018

Filed:

May. 06, 2013
Applicant:

Merck Sharp & Dohme Corp., Rahway, NJ (US);

Inventors:

Hussam Hisham Shaheen, Lebanon, NH (US);

Dongxing Zha, Lebanon, NH (US);

Assignee:

Merck Sharp & Dohme Corp., Rahway, NJ (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C40B 40/02 (2006.01); C12N 15/10 (2006.01); G01N 33/68 (2006.01); C07K 16/00 (2006.01); C07K 16/40 (2006.01); C07K 14/39 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1055 (2013.01); C07K 14/39 (2013.01); C07K 16/00 (2013.01); C07K 16/40 (2013.01); G01N 33/6854 (2013.01); G01N 33/6857 (2013.01); C07K 2317/41 (2013.01); C07K 2317/52 (2013.01); C07K 2317/56 (2013.01); C07K 2319/02 (2013.01); C07K 2319/035 (2013.01); C40B 40/02 (2013.01); G01N 2440/38 (2013.01);
Abstract

The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. Embodiments of the invention provide a system in which a bait complexed with a monovalent antibody fragment can be captured prior to secretion in a host cell by virtue of surface displaying an antibody light chain and utilizing the covalent interaction of this light chain with the heavy chain of an antibody molecule that is co-expressed in the same host. Polypeptides, polynucleotides and host cells useful for making the antibody display system are also provided along with methods of using the system for identifying antibodies that bind specifically to an antigen of interest.


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