The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 13, 2018

Filed:

Sep. 12, 2014
Applicant:

Bgi Shenzhen Co., Limited, Shenzhen, CN;

Inventors:

Chunyu Geng, Shenzhen, CN;

Dennis G. Ballinger, Menlo Park, CA (US);

Yanyan Zhang, Shenzhen, CN;

Shujin Fu, Shenzhen, CN;

Lingyu He, Shenzhen, CN;

Wenwei Zhang, Shenzhen, CN;

Hui Jiang, Shenzhen, CN;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12N 15/10 (2006.01); B01J 19/00 (2006.01); C40B 60/14 (2006.01);
U.S. Cl.
CPC ...
C12N 15/10 (2013.01); B01J 19/0046 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6811 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6853 (2013.01); C40B 60/14 (2013.01); B01J 2219/00529 (2013.01); C12Q 2565/537 (2013.01);
Abstract

Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5'-end nucleotide of the first strand has a phosphate group, and the 3′-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5′-end nucleotide of the second strand does not have a phosphate group, and the 3′-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.


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