The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 06, 2018

Filed:

Jun. 04, 2015
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Matthew B. Francis, Berkeley, CA (US);

Katherine J. Mackenzie, Berkeley, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01N 65/00 (2009.01); C12P 19/14 (2006.01); C12P 19/12 (2006.01); C12N 11/08 (2006.01); C08F 220/56 (2006.01); C12P 19/02 (2006.01); C12N 9/16 (2006.01); C12N 9/20 (2006.01); C12N 9/24 (2006.01); C12N 9/26 (2006.01); C12N 9/42 (2006.01); C12N 9/96 (2006.01);
U.S. Cl.
CPC ...
C12P 19/14 (2013.01); C08F 220/56 (2013.01); C12N 9/16 (2013.01); C12N 9/20 (2013.01); C12N 9/2402 (2013.01); C12N 9/248 (2013.01); C12N 9/2411 (2013.01); C12N 9/2437 (2013.01); C12N 9/2477 (2013.01); C12N 9/96 (2013.01); C12N 11/08 (2013.01); C12P 19/02 (2013.01); C12P 19/12 (2013.01); C12P 2203/00 (2013.01);
Abstract

Provided herein are methods for enhancing enzymatic activity using certain polymers that may be optionally attached to an enzyme. The polymers may be thermally-responsive polymers, including poly N-isopropylacrylamide or poly N-isopropylmethacrylamide. The polymer may also be a copolymer with at least two different monomer residues. The monomer residues may have a structure of formula (I): wherein R, Rand Rare as described herein. Examples of such monomer residues may include N-isopropylacrylamide (NIPAm) or N-isopropylmethacrylamide (NIPMa). The polymer may include additional monomer residues, such as aminooxy-bearing methacrylamide monomer residues that can be modified to vary the lower critical solution temperature (LCST) of the polymer.


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