The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 30, 2018

Filed:

Oct. 03, 2014
Applicant:

Virovek, Inc., Hayward, CA (US);

Inventor:

Haifeng Chen, Piedmont, CA (US);

Assignee:

VIROVEK, INC., Hayward, CA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 15/86 (2006.01); C12N 7/00 (2006.01); C12N 15/85 (2006.01); C07K 14/005 (2006.01);
U.S. Cl.
CPC ...
C12N 15/86 (2013.01); C07K 14/005 (2013.01); C12N 7/00 (2013.01); C12N 15/85 (2013.01); C12N 2750/00043 (2013.01); C12N 2750/00051 (2013.01); C12N 2750/14151 (2013.01); C12N 2750/14152 (2013.01); C12N 2800/105 (2013.01); C12N 2800/50 (2013.01); C12N 2830/42 (2013.01); C12N 2840/20 (2013.01);
Abstract

The present teachings disclose nucleic acid cassettes for expressing in an insect cell a plurality of polypeptides encoded by a gene comprising overlapping open reading frames (ORFs). A cassette comprises, in 5' to 3′ order, a) a first insect cell-operable promoter, b) a 5′ portion of a gene comprising a first ORF of the gene, c) an intron comprising a second insect cell-operable promoter, and d) a 3′ portion of the gene comprising at least one additional ORF. Vectors and insect cells comprising the cassettes are also disclosed, as well as methods for production of recombinant adeno-associated virus in insect cells using the cassettes.


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