The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 16, 2018
Filed:
Jul. 23, 2014
Suzhou Helmen Precision Instruments Co., Ltd., Suzhou, Jiangsu, CN;
Suzhou Helmen Precision Instruments Co., Ltd., Suzhou, CN;
Abstract
The present invention relates to a method for detecting time-resolved fluorescence based on a principle of phase balanced frequency multiplication modulation. A stimulating light source modulated by using a baseband signal acts on a to-be-measured target to trigger fluorescence, so that the fluorescence intensifies and decays periodically; then, a frequency-doubled square signal is used to control a sampling period and divide an ascending period of the fluorescence into two and a decay period of the fluorescence into two; after independent sampling is performed separately, sampling differences of the two parts are separately calculated and then added to obtain an intensity representative value of a fluorescence signal and to obtain a concentration value of the to-be-measured target. The method in the present invention can not only likewise cancel fluorescence interference of a substrate in a sample, but also can cancel ambient bias light, power-frequency interference of a spatial electromagnetic wave or other signals, and therefore improves signal intensity in fluorescence measurement on the detection sample, has an advantage that cannot be accomplished in a conventional time-resolved fluorescence method, and can be applied in fluorescence intensity detection of a target in fields such as biology, chemistry, and medicine.