The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 16, 2018
Filed:
Oct. 28, 2013
Applicant:
Enzynomics Co. Ltd., Daejeon, KR;
Inventors:
Yong Keol Shin, Daejeon, KR;
Yunil Suh, Daejeon, KR;
Min Young Lee, Daejeon, KR;
Ah Rong Jeong, Daejeon, KR;
Assignee:
ENZYNOMICS CO. LTD., Daejeon, KR;
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 15/10 (2006.01); C12N 15/64 (2006.01); C07H 21/04 (2006.01); C12N 9/12 (2006.01); C12N 15/65 (2006.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C07H 21/04 (2013.01); C12N 9/12 (2013.01); C12N 15/10 (2013.01); C12N 15/64 (2013.01); C12N 15/65 (2013.01);
Abstract
Disclosed are methods and kits for controlling the length of overhangs at the ends of double stranded DNA based on a competitive inhibition. Further provided are ligase-independent methods for joining the two ends of DNA strands using the same. The present methods efficiently control the overhangs of ds DNA. This user-controlled overhangs supply a tool for efficient ligation in a ligase independent way and can be advantageously used in DNA recombinant technology such as cloning gene or DNA fragments of interest or PCR products.