The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 16, 2018
Filed:
Jul. 08, 2016
Jiangnan University, Wuxi, Jiangsu, CN;
Long Liu, Wuxi, CN;
Jian Chen, Wuxi, CN;
Guocheng Du, Wuxi, CN;
Jianghua Li, Wuxi, CN;
Yang Gu, Wuxi, CN;
Yang Song, Wuxi, CN;
Jieying Deng, Wuxi, CN;
Yawen Zhao, Wuxi, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
Abstract
The invention provides an effective method for improving N-acetylglucosamine (GlcNAc) production by engineeredDeletion of phosphoenolpyruvate carboxykinase encoding gene pckA and encoding pyruvate kinase gene pyK in recombinant GlcNAc-producing strain BSGNK-PxylA-glmS-P43-GNA1 (BSGNK) is first performed to enhance GlcNAc production, followed by overexpression of pyruvate carboxylase encoding gene pycA for facilitating cell growth. Finally, the GlcNAc production of the recombinant strain BPTS3 reached to 11.3 g/L, which was 1.84-fold of BSGNK. This method can be used for improve cellular property of engineeredfor GlcNAc production, which can be further applied to industrial production of GlcNAc.