The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 16, 2018

Filed:

Jul. 25, 2014
Applicant:

Ube Industries, Ltd., Ube-shi, Yamaguchi, JP;

Inventors:

Masahiko Hagihara, Ube, JP;

Tetsuo Kawaguchi, Ube, JP;

Kousuke Baba, Ube, JP;

Motohisa Shimizu, Ube, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12M 1/12 (2006.01); C12N 1/16 (2006.01); C12N 1/20 (2006.01); C12N 11/08 (2006.01); C08L 79/08 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0068 (2013.01); C12M 25/02 (2013.01); C12N 1/16 (2013.01); C12N 1/20 (2013.01); C12N 11/08 (2013.01); C08L 79/08 (2013.01); C12N 2533/30 (2013.01);
Abstract

The present invention relates to a cell culturing method and cell culturing apparatus and kit for use therewith. The method includes applying cells on a porous polyimide film and culturing. One embodiment includes a process for sowing cells on the surface of the film. Another embodiment includes a process for placing a cell suspension on the dried surface of the film and leaving the film undisturbed, moving the film to promote liquid efflux, or stimulating a portion of the surface to entangle the cell suspension into the film, and then retaining the cells in suspension inside the film while allowing moisture to flow out. Another embodiment includes a process for moistening one or both surfaces of the film with a cell culture solution or sterilized liquid, loading a cell suspension on the moistened film, retaining the cells in suspension inside the film, and allowing moisture to flow out.


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